Overview
Jehangir completed an undergraduate BSc. (Hons.) degree in Physics at St. Stephen's College, University of Delhi, before joining Trinity College, Cambridge to study Parts II and III of the Natural Sciences Tripos (Physics). He continued at Trinity and at the Cavendish Laboratory in Cambridge for his PhD under the supervision of Prof. Ulrich Keyser, where he focused on understanding antibiotic transport across biomimetic membrane systems. This is of particular relevance for Gram-negative bacteria, whose double membrane cell envelope is a formidable permeability barrier that protects these cells from small molecule antibiotics. Understanding the physical processes governing Gram-negative drug permeation is a crucial challenge facing antibiotic developers, who are working to counteract the alarming spread of antibiotic resistance in bacterial pathogens across the globe. Jehangir continued this research as a PDRA in Prof. Keyser's group, and was also a Research Fellow at Clare Hall, Cambridge.
Besides studying antibiotic permeation, Jehangir is also part of a multi-centre consortium which includes participants from the National Physical Laboratory, the STFC Hartree Centre, IBM Research and the Universities of Cambridge and Exeter, that is developing a new pipeline of polypeptide antibiotics. These peptides circumvent the permeability barrier of Gram-negatives by directly attacking and lysing bacterial membranes. Jehangir is involved in the development of assays to quantify the efficacy and safety of these new drugs.
Jehangir's technical expertise is in the development of microfluidic technologies for quantitative biology. His current projects include:
- Quantifying antibiotic accumulation label-free in Gram-negative bacteria in well-defined microenvironments. Collaboration with Dr. Stefano Pagliara, Prof. Krasimira Tsaneva-Atanasova, Dr. Margaritis Voliotis and Dr. Jeremy Metz (LSI, Exeter) and Prof. Matthew Cooper and Dr. Mark Blaskovich (University of Queensland).
- Quantifying antibiotic permeation across specific lipid and protein pathways using biomimetic lipid vesicles. Collaboration with Prof. Ulrich Keyser (Cambridge), Prof. Mathias Winterhalter (Jacobs University, Bremen) and Prof. Christopher Dowson (Warwick).
- Developing assays to quantify the efficacy and safety of novel polypeptide antibiotics. Collaboration with Dr. Max Ryadnov (NPL, Teddington), Prof. Ulrich Keyser (Cambridge), Dr. Stefano Pagliara (LSI, Exeter), IBM Research, the STFC Hartree Centre, with further involvement from industry partners.
- Studying the biophysical aspects of indole signalling and its effects on bacterial metabolism. Collaboration with Dr. David Summers (Dept. of Genetics, Cambridge).
Jehangir joined the Living Systems Institute in Exeter in February 2019 as an Industry Research Fellow. In addition to his experimental research, he is working towards the development of research collaborations with pharmaceutical and biotechnology partners in the field of antibiotic testing and development.
Research Themes: Membrane transport, antibiotic resistance, microfluidics, label-free optical detection, bacterial signalling.
LinkedIn profile: https://www.linkedin.com/in/jehangir-cama-7a451b16a/
Google Scholar: https://scholar.google.co.uk/citations?user=mF5LzJQAAAAJ&hl=en
Publications
Copyright Notice: Any articles made available for download are for personal use only. Any other use requires prior permission of the author and the copyright holder.
| 2022 | 2021 | 2020 | 2019 | 2016 | 2015 | 2014 | 2013 |
2022
- Ives L, Pace A, Bor F, Jing Q, Wade T, Cama J, Khanduja V, Kar-Narayan S. (2022) Conformable and robust microfluidic force sensors to enable precision joint replacement surgery, Materials & Design, volume 219, DOI:10.1016/j.matdes.2022.110747.
- Al Nahas K, Fletcher M, Hammond K, Nehls C, Cama J, Ryadnov MG, Keyser UF. (2022) Measuring Thousands of Single-Vesicle Leakage Events Reveals the Mode of Action of Antimicrobial Peptides, Anal Chem, volume 94, no. 27, pages 9530-9539, DOI:10.1021/acs.analchem.1c03564. [PDF]
- Cama J, Al Nahas K, Fletcher M, Hammond K, Ryadnov MG, Keyser UF, Pagliara S. (2022) An ultrasensitive microfluidic approach reveals correlations between the physico-chemical and biological activity of experimental peptide antibiotics, Scientific Reports, volume 12, no. 1, DOI:10.1038/s41598-022-07973-z.
2021
- Cama J, Al Nahas K, Fletcher M, Hammond K, Ryadnov MG, Keyser UF, Pagliara S. (2021) An ultrasensitive microfluidic approach reveals correlations between the physico-chemical and biological activity of experimental peptide antibiotics, DOI:10.1101/2021.09.08.459503. [PDF]
- Ives L, Pace A, Bor F, Jing Q, Wade T, Cama J, Khanduja V, Kar-Narayan S. (2021) Conformable and robust force sensors to enable precision joint replacement surgery, DOI:10.1101/2021.08.19.456934. [PDF]
- Nahas KA, Fletcher M, Hammond K, Nehls C, Cama J, Ryadnov MG, Keyser UF. (2021) Measuring thousands of single vesicle leakage events reveals the mode of action of antimicrobial peptides, DOI:10.1101/2021.08.13.455434. [PDF]
- Goode O, Smith A, Zarkan A, Cama J, Invergo BM, Belgami D, Caño-Muñiz S, Metz J, O’Neill P, Jeffries A. (2021) Persister Escherichia coli Cells Have a Lower Intracellular pH than Susceptible Cells but Maintain Their pH in Response to Antibiotic Treatment, mBio, volume 12, no. 4, DOI:10.1128/mbio.00909-21. [PDF]
- Hammond K, Cipcigan F, Al Nahas K, Losasso V, Lewis H, Cama J, Martelli F, Simcock PW, Fletcher M, Ravi J. (2021) Switching Cytolytic Nanopores into Antimicrobial Fractal Ruptures by a Single Side Chain Mutation, ACS Nano, DOI:10.1021/acsnano.1c00218. [PDF]
- Jing Q, Pace A, Ives L, Husmann A, Catic N, Khanduja V, Cama J, Kar-Narayan S. (2021) Aerosol-jet-printed, conformable microfluidic force sensors, Cell Reports Physical Science, volume 2, pages 100386-100386, DOI:10.1016/j.xcrp.2021.100386. [PDF]
- Cama J, Leszczynski R, Tang PK, Khalid A, Lok V, Dowson CG, Ebata A. (2021) To Push or To Pull? In a Post-COVID World, Supporting and Incentivizing Antimicrobial Drug Development Must Become a Governmental Priority, ACS Infectious Diseases, DOI:10.1021/acsinfecdis.0c00681. [PDF]
2020
- Jing Q, Pace A, Ives L, Husmann A, Catic N, Khanduja V, Cama J, Kar-Narayan S. (2020) Aerosol-Jet Printed Conformable Microfluidic Force Sensors, DOI:10.2139/ssrn.3732370.
- Cama J, Pagliara S. (2020) Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials, Polypeptide Materials, Humana, New York, NY, 237-253, DOI:10.1007/978-1-0716-0928-6_16. [PDF]
- Cama J, Voliotis M, Metz J, Smith A, Iannucci J, Keyser UF, Tsaneva K, Pagliara S. (2020) Single-cell microfluidics facilitates the rapid quantification of antibiotic accumulation in Gram-negative bacteria, Lab on a Chip, DOI:10.1039/D0LC00242A.
- Schaich M, Sobota D, Sleath H, Cama J, Keyser UF. (2020) Characterization of lipid composition and diffusivity in OLA generated vesicles, Biochimica et Biophysica Acta (BBA) - Biomembranes, volume 1862, no. 9, pages 183359-183359, article no. 183359, DOI:10.1016/j.bbamem.2020.183359. [PDF]
- Ćatić N, Wells L, Al Nahas K, Smith M, Jing Q, Keyser UF, Cama J, Kar-Narayan S. (2020) Aerosol-jet printing facilitates the rapid prototyping of microfluidic devices with versatile geometries and precise channel functionalization, Applied Materials Today, volume 19, pages 100618-100618, article no. 100618, DOI:10.1016/j.apmt.2020.100618. [PDF]
2019
- Schaich M, Cama J, Al Nahas K, Sobota D, Sleath H, Jahnke K, Deshpande S, Dekker C, Keyser UF. (2019) An Integrated Microfluidic Platform for Quantifying Drug Permeation across Biomimetic Vesicle Membranes, Molecular Pharmaceutics, volume 16, pages 2494-2501, article no. 6, DOI:10.1021/acs.molpharmaceut.9b00086. [PDF]
- Cama J, Henney A, Winterhalter M. (2019) Breaching the Barrier: Quantifying Antibiotic Permeability across Gram-Negative Bacterial Membranes, Journal of Molecular Biology, DOI:10.1016/j.jmb.2019.03.031.
- Al Nahas K, Cama J, Schaich M, Hammond K, Deshpande S, Dekker C, Ryadnov MG, Keyser UF. (2019) A microfluidic platform for the characterisation of membrane active antimicrobials, Lab on a Chip, DOI:10.1039/c8lc00932e. [PDF]
- Zarkan A, Cano-Muniz S, Zhu J, Al Nahas K, Cama J, Keyser UF, Summers DK. (2019) Indole Pulse Signalling Regulates the Cytoplasmic pH of E. coli in a Memory-Like Manner, Scientific Reports, volume 9, DOI:10.1038/s41598-019-40560-3.
2016
- Cama J, Schaich M, Al Nahas K, Hernández-Ainsa S, Pagliara S, Keyser UF. (2016) Direct Optofluidic Measurement of the Lipid Permeability of Fluoroquinolones, Scientific Reports, volume 6, DOI:10.1038/srep32824.
- Purushothaman S, Cama J, Keyser UF. (2016) Dependence of norfloxacin diffusion across bilayers on lipid composition, Soft Matter, volume 12, no. 7, pages 2135-2144, DOI:10.1039/c5sm02371h.
2015
- Cama J, Bajaj H, Pagliara S, Maier T, Braun Y, Winterhalter M, Keyser UF. (2015) Quantification of Fluoroquinolone Uptake through the Outer Membrane Channel OmpF of Escherichia coli, J Am Chem Soc, volume 137, no. 43, pages 13836-13843, DOI:10.1021/jacs.5b08960. [PDF]
2014
- Gaimster H, Cama J, Hernandez-Ainsa S, Keyser UF, Summers DK. (2014) The Indole Pulse: A New Perspective on Indole Signalling in Escherichia coli, PLOS ONE, volume 9, no. 4, article no. ARTN e93168, DOI:10.1371/journal.pone.0093168. [PDF]
- Cama J, Chimerel C, Pagliara S, Javer A, Keyser UF. (2014) A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes, Lab on a Chip, volume 14, no. 13, pages 2303-2308, DOI:10.1039/c4lc00217b.
2013
- Chimerel C, Ainsa SMH, Cama J, Summers DK, Keyser UF. (2013) Bacterial Signal Indole Modifies the Physicochemical Properties of Lipid Membranes, BIOPHYSICAL JOURNAL, volume 104, no. 2, pages 251A-251A, DOI:10.1016/j.bpj.2012.11.1413. [PDF]